Acta Sci. Pol., Technol. Aliment. 8(3) 2009, 37-46
ACTA
ISSN 1644-0730 (print) ISSN 1889-9594 (online)
EFFECT OF PULLULAN COATING ON INHIBITION
OF CHOSEN MICROORGANISMS GROWTH
Anna Chlebowska-Śmigiel, Małgorzata Gniewosz
Warsaw University of Life Sciences SGGW
Background. Pullulan is a water-soluble polysaccharide produced by fungi Aureobasi-
dium pullulans. This glucan was applied for coating of food products. The aim of this
study was obtaining of a pullulan coating and checking its effect on growth of microor-
ganisms responsible for spoilage of food.
Materials and methods. Pullulan produced by the white mutant A. pullulans B-1 was ap-
plied. Permeability of oxygen and carbon dioxide through film produced from 10% water
solution of pullulan was checked as well as degree of inhibition of chosen microorgan-
isms through pullulan coating formed on surface of growth s media.
Results. Low permeability of gases through pullulan film and a considerable growth s
limitation of all tested microorganisms were found. A total growth s inhibition of 21 from
36 tested strains and a partial growth s limitation of the remaining 15 strains was ob-
served. The inhibitory effect was diverse and it was from 63 to 100%.
Conclusions. These results proved that pullulan coating applied in these tests revealed big
barrier characteristics in relation to oxygen and carbon dioxide, which had effect upon
growth s inhibition of most of the tested microorganisms, responsible for spoilage
of food.
Key words: edible coating, pullulan, microbiological contamination of food
INTRODUCTION
Progressing social-economic development, increasing requirements of consumers
as well as bigger and bigger demand on portioned and processed to a minimum degree
food [Cichoń and Miśniakiewicz 2000], functional and convenience [Czapski 2002],
intention to diversify and to make product more attractive, on the other hand to prolong
offered product s durability time, it requires from food manufacturers not only changes
in technological processes, but also looking for new methods of food packing. Into such
© Copyright by Wydawnictwo Uniwersytetu Przyrodniczego w Poznaniu
Corresponding author Adres do korespondencji: Dr hab. Małgorzata Gniewosz, Department
of Biotechnology, Microbiology and Food Evaluation of Warsaw University of Life Sciences
SGGW, Nowoursynowska 159 C, 02-767 Warsaw, Poland, e-mail: malgorzata_gniewosz@sggw.pl
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38 A. Chlebowska-Åšmigiel, M. Gniewosz
methods coating of raw materials and food products with edible coatings can be in-
cluded [Debeaufort et al. 1998]. Safe for human health and for environment, produced
from biodegradable materials they are an alternative for plastic packagings.
The main purpose of food s packing is limitation of a negative effect, which envi-
ronment has upon a product and preserving product s high quality during the whole
period of its usefulness for consumption. Edible coatings should delay loss of moisture,
limit migration of fatty compounds and solved substances, retain volatile components
and be a carrier of supplementary substances [Krochta and De Mulder-Johnston 1997,
Petersen et al. 1999, Tharanathan 2003]. Basic components for producing of edible
coatings are fats, proteins and polysaccharides [Tharanathan 2003]. Coatings formed
from lipids, owing to their hydrophobic characteristics are a good barrier for water va-
pour, inhibiting its evaporating out of product, but they are characterised by low me-
chanic properties and high permeability of oxygen [Kester and Fennema 1989]. Then
coatings produced of proteins or polysaccharides have good mechanic characteristics
and at low humidity of environment they inhibit access of oxygen to product, still they
are generally hydrophilic, they easily absorb water and are characterised by high perme-
ability of water vapour [Li and Chen 2000, Gontard et al. 1996]. In order to improve
characteristics of produced films and edible coatings instead of one component a mix-
ture of several substances is applied [Guilbert et al. 1996, Diab et al. 2001, Petersen
et al. 1999]. Most often hydrocolloids are combined (proteins, polysaccharides), which
form a crosslinked, integral and coherent matrix with hydrophobic compounds (lipids),
which mainly improve barrier characteristics of film in relation to water vapour. Shih
[1996] found, that film formed from rice protein and pullulan mixed in the ratio of 1:1
was characterised by better mechanic properties (among others it had higher tear resis-
tance) in relation to films made from single components.
Pullulan is a polysaccharide soluble in water, produced by fungi Aureobasidium pul-
lulans [Yuen 1974, Simon et al. 1995]. It is not toxic for people and animals. It is low-
caloric and totally biodegradable [Leathers 2003, Lee et al. 2001]. Pullulan reveals good
adhesive characteristics, that enables it to be applied for coating of food products. Coat-
ings made of pullulan are colourless, odourless and have no taste. They can be very thin,
even of thickness of about 0.01 mm [Yuen 1974]. Pullulan coatings can be applied for
prolongation of fruit and vegetables durability. Coating formed on strawberries and
kiwi fruit had an advantageous effect upon maintaining of firmness, colour and upon
reduction of fruit weight s decrease [Diab et al. 2001]. A similar effect was obtained
applying to kiwi fruit a coating from a mixture of pullulan, soya protein, glycerol and
stearic acid. The coated fruit stored at ambient temperature underwent softening process
three times more slowly than uncoated fruit [Xu et al. 2001].
There are no direct data concerning possibility of inhibition of micro bacterial
growth by pullulan coatings. That is why the purpose of this work was obtaining of
a pullulan coating and checking in model tests its effect on growth of microorganisms,
which are the reason of food s decay.
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Effect of pullulan coating on inhibition of chosen microorganisms growth 39
MATERIALS AND METHODS
Biological material, conditions of culture and production of pullulan
In the tests a white mutant of the fungus Aureobasidium pullulans B-1 was used,
which had been selected after an associated mutagenisation (UV and ethyleneimine)
of a wild strain A. pullulans A.p.-3. Mutant B-1 in comparison with the natural parental
strain is characterised by higher production of pullulan and by lack of synthesis of
melanin compounds, contaminating raw pullulan preparation [Gniewosz et al. 1999,
Gniewosz and Duszkiewicz-Reinhard 2008]. The strain was cultivated on substrate of
the following composition (g/L): saccharose 60, K2HPO4 7.5, NaCl 1.5, (NH4)2 SO4
0.72, MgSO4·7H2O 0.4, yeast extract 0.4, pH was 6.0 Ä…0.5 [Gniewosz et al. 1997]. The
culture was cultivated at the temperature of 28°C for 48 hours in a SM-30/C shaker,
(Otto Gmbh, Germany) of 200 rpm. Next 1 mL inoculum was transferred into fresh
liquid substrate and culture of the fungus was carried out in the same conditions as pre-
viously (28°C, 200 rpm) for 96 hours. After that time of culture biomass of the fungus
was centrifuged at 18 000 x g for 20 minutes (Centrifuge 5804 R, Eppendorf, Germany).
Next pullulan was precipitated from the post-culture liquid by use of 96% ethanol,
which was added to supernatant in the ratio of 1:1 (v/v). The precipitated pullulan was
centrifuged at 11 000 x g for 10 minutes, and then it was subjected to purification after
the method of Roukas and Biliaderis [1995]. Pullulan was dried at the temperature of
55°C and ground in a mill.
Determination of oxygen and carbon dioxide s permeability through pullulan film
The film was obtained from 10% water solution of pullulan, which in quantities of
10, 15 and 20 mL was poured onto sterile Petri plates of 15 cm diameter. In order to dry
partly the films the plates were placed in a laminar chamber under blow of sterile air for
2 hours. Next the plates were left until the film was completely dried at room tempera-
ture (22°C) and at a relative humidity of air 55%. Next the films were carefully taken
off the glass surface and carried over into a chamber of constant relative humidity of air
(RH 50%) and at the temperature of 22°C for three days. After the films had been dried,
they were measured by means of 122 DM apparatus (Mercer) and fragments were cut
out of the thickness respectively 15 Ä…3, 25 Ä…5 and 40 Ä…5 µm.
Determination of oxygen s permeability of films prepared in such a way was carried
out in accordance with ASTM D 3985-05 norm by means of OX-TRAN 100 apparatus
with a coulometric detector. As gas carrier nitrogen containing 3% hydrogen was applied.
The test was carried out at the temperature of 23 Ä…2°C and at relative humidity 0%. De-
termination of carbon dioxide s permeability was carried out by means of Permatran C-
200 (Mocon, USA) with a sensor in infrared range (wave length 4.3 µm). As gas carrier
dry nitrogen was used. The test was carried out at the temperature of 23°C and at rela-
tive humidity from 50 to 1%. Both determinations were carried out in the Central Re-
search and Development Center (COBRO) in Warsaw.
Test strains of fungi and bacteria
In the tests the following pure cultures were used:
Acta Scientiarum Polonorum, Technologia Alimentaria 8(3) 2009
40 A. Chlebowska-Åšmigiel, M. Gniewosz
moulds: Alternaria alternata, Aspergillus flavus, Aspergillus glaucus, Aspergillus
niger ATCC 9142, Aspergillus oryzae, Geotrichum candidum, Botritis cinerea
E 92, Cladosporium herbarum, Fusarium avenaceum F VIII, Fusarium species
F VII, Monilia species, Mucor mucedo KKP 461, Penicillium notatum E 30, Peni-
cillium roqueforti E 31, Rhizopus arrhizus ATCC 11145, Rhizopus nigricans
KKP 484, Rhizopus oryzae M/180, Trichoderma harzianum KKP 534,
yeasts: Candida mycoderma, Candida utilis ATCC 9950, Endomyces magnusi,
Hansenula anomala R 26, Kluyveromyces fragilis R 11, Pichia jadinii, Rhodoto-
rula rubra Rh VIII, Saccharomyces cerevisiae ATCC 2366, Schizosaccharomyces
pombe R 25, Torulopsis utilis R 10,
bacteria: Bacillus subtilis ATCC 6650, Citrobacter freundii ATCC 8090, Escheri-
chia coli ATCC 25922, Lactobacillus plantarum ATCC 4080, Micrococcus luteus
ATCC 9341, Pseudomonas fluorescens, Sarcina sp., Tetracoccus sp.
All the strains came from the Collection of Pure Cultures of Department of Biotech-
nology and Food Microbiology of Warsaw University of Life Sciences (SGGW). The
strains of fungi were stored on YPD medium, and bacteria strains on Nutrient Agar
medium or MRS broth (Lb. plantarum ATCC 4080) at the temperature of 4°C.
Methods of carrying out of tests strains culture under pullulan coating
Inoculum of microorganisms (105-106 CFU/mL) was carried over on Petri plates and
a corresponding medium was poured on them. On dispersion of moulds spores
Sabourand Agar medium (BTL, Poland) was poured, on dispersion of yeasts YPD me-
dium of the composition (g/L): glucose 10, peptone 20, yeast extract 10, agar 25 was
poured, and on dispersion of bacteria Nutrient Agar medium or in case of Lb. plantarum
MRS medium (Merck, Germany) was poured. On surface of each medium 1 mL of 10%
water solution of pullulan was carried over. For quicker drying of the pullulan coating
the plates were placed for about 1 hour in a laminar chamber with a constant flow of
sterile air. In parallel control plates were prepared (uncovered with pullulan coating).
After the coating has got set, the plates with the pullulan coating and without it were
incubated at the temperature of 28°C or 37°C for 24-96 hours (depending on the strain).
After that period of time the grown up colonies of microorganisms were counted.
The test was carried out in three series. On the base of the obtained results the ratio
of the number of microorganisms colonies grown up on the plates with the pullulan
coating to the number of colonies grown up on the control plates was calculated. Thus
obtained results were used for calculation of inhibition degree of the tested bacteria s
growth by the pullulan coating.
DISCUSSION AND RESULTS
Testing of permeability degree of oxygen and carbon dioxide through pullulan film
Control of concentration level of oxygen and carbon dioxide around food products is
a well known method of prolonging food s durability. Oxygen accelerates irreversible
processes of majority of dyestuffs and vitamins degradation, and it is one of the reasons
of unsaturated lipids auto oxidation as well [Wilska-Jeszka 2002, Drozdowski 2002].
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Effect of pullulan coating on inhibition of chosen microorganisms growth 41
That is why high barrier characteristics of coatings in relation to gases is very much
desirable.
The formed pullulan film was subjected to testing of oxygen and carbon dioxide s
permeability. Permeability of oxygen was between 570-6100 cm3/m2 of film during
24 hours at the pressure of 0.1013 MPa, at the ambient relative humidity of 0% and it
was closely connected with film s thickness. The thicker pullulan film, the lower per-
meability of oxygen was observed (Table 1). At a mean thickness of pullulan film of
25 µm oxygen s permeability was at the level of 1600 cm3/m2/24 hours, which testifies
to the fact, that pullulan film is a good barrier for oxygen. The obtained results are com-
parative with oxygen s permeability through polypropylene and polyethylene films,
whose permeability of this gas was respectively 1600 cm3/m2/24 hours and 1400-8000
cm3/m2/24 hours [Janicki and Ćwiek-Ludwicka 2003].
Table 1. Barrier specificity of pullulan film
RH Thickness of film Results of designations
Kind of research
% µm Ä…SD cm3/m2·24 h·0.1 MPa
Permeability of oxygen 0 15 Ä…5 6 100
25 Ä…5 1 600
40 Ä…5 570
Permeability of carbon dioxide 50 25 Ä…5 < 100
1 < 10
Literature data make known, that permeability of oxygen and carbon dioxide of
various materials depends not only on films thickness, but on values of environment s
relative humidity as well. Gontard and al. [1996] observed, that at low relative humidity
value edible film of wheat gluten revealed low permeability of oxygen and carbon diox-
ide, respectively 1.24 and 7.4 amol/(Pa·m·s). At humidity over 60% permeability of
these both gases increased rapidly up to the values of 1290 and 36 700 amol /(Pa·m·s).
In the tests carried out in the present work a similar tendency was observed. With drop
of relative humidity permeability of coating dropped in relation to carbon dioxide.
At coating s thickness of 25 µm and with humidity drop from 50% to 1% reduction
of permeability of CO2 from 100 cm3/m2 · 24 hours · 0.1 MPa to 10 cm3/m2 · 24 hours ·
0.1 MPa was observed.
Effect of pullulan coating on speed of growth of chosen moulds strains
On the base of the obtained results for each of the tested moulds strains degree of
growth s inhibition under pullulan coating in relation to growth on control substrate
(without any coating) was calculated. The obtained results were presented in the Fig-
ure 1.
In case of all the 18 tested moulds strains inhibitory effect of applied pullulan coat-
ing was observed. The degree of growth s inhibition was diverse for various strains and
it was from 54% to 100%. 12 tested moulds did not reveal any growth on the substrate
Acta Scientiarum Polonorum, Technologia Alimentaria 8(3) 2009
42 A. Chlebowska-Åšmigiel, M. Gniewosz
100
90
80
70
60
50
40
30
20
10
0
1 3 5 7 8 9 11 12 15 16 17 18 6 4 13 14 10 2
Mould strains
Fig. 1. The degree of growth s inhibition of chosen moulds strains through pul-
lulan coating: 1 Alternaria alternata, 3 Aspergillus glaucus, 5
Aspergillus oryzae, 7 Cladosporium herbarum, 8 Fusarium avena-
ceum F VIII, 9 Fusarium sp., 11 Monilia sp., 12 Mucor mucedo
KKP 461, 15 Rhizopus arrrhizus ATCC 11145, 16 Rhizopus nigricans
KKP 484, 17 Rhizopus oryzae M/180, 18 Trichoderma harzianum
KKP 534, 6 Botritis cinerea E 92, 4 Aspergillus niger ATCC 9142,
13 Penicillium notatum E 30, 14 Penicillium roqueforti E 31, 10 Ga-
lactomyces geotrichum, 2 Aspergillus flavus
with carried on pullulan coating, which makes two third of all tested strains. In this
group there were: Alternaria alternata, Cladosporium herbarum, Monilia sp., Trichode-
rema harzianum KKP 534 and two species of each of the genus Aspergillus, Rhizopus
and Fusarium. The least sensitive to presence of the pullulan coating proved to be the
strain Aspergillus flavus, still its growth under the coating was inhibited in 54% as well.
Xu et al. [2001] testing coatings on base of rice protein with supplement of pullulan
found, that presence of this compound in the coating had an advantageous effect upon
increasing of the coating s barrier characteristics in relation to oxygen. Similar results
were obtained by Yuen [1974] and Roller and Dea [1992], who suggest that the mecha-
nism of pullulan coating s activity resolves itself into a mechanic severance of oxygen s
access from environment. Because moulds are organisms that require presence of oxy-
gen for their growth, and the formed in the present tests pullulan coating was character-
ised by a big ability of limitation of gas exchange between environment and the coated
material, this phenomenon was probably the reason of mould s growth inhibition.
In the carried out tests limitation of six other moulds growth was observed as well.
They were: Botritis cinerea E 92, Penicillium notatum E 30, Aspergillus niger ATCC
9142, Penicillium roqueforti E 31, Galactomyces geotrichum and Aspergillus flavus.
A significant factor limiting effect of pullulan coating on growth of these moulds was
delay of sporification. It was probably brought about by the fact, that colonies of the
moulds on plates with the carried on pullulan coating were appearing with 24 hours
delay in relation to growth of the moulds on the control plates.
Zhang and Quantick [1998] testing effect of chitosan coating upon prolongation of
fresh raspberries and strawberries durability period found, that application of this coat-
ing limited growth of moulds Botritis cinerea and Rhizopus sp. The inhibitory effect
was contained within the range from 40 to 62%.
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Effect of pullulan coating on inhibition of chosen microorganisms growth 43
Effect of pullulan coating on speed of growth of chosen yeast strains
On base of the obtained results for each of the tested yeast strains degree of growth s
inhibition on plates with pullulan coating in relation to growth on control plates was
determined. The obtained results were presented in the Figure 2.
100
90
80
70
60
50
40
30
20
10
0
3 5 6 7 9 10 1 8 4 2
Yeast strains
Fig. 2. The degree of growth s inhibition of chosen yeast strains through pullu-
lan coating: 3 Endomyces magnusi, 5 Kluyveromyces fragilis R11,
6 Pichia jadinii, 7 Rhodotorula rubra Rh VIII, 9 Schizosaccharomy-
ces pombe R25, 10 Torulopsis utilis R10, 1 Candida mycoderma,
8 Saccharomyces cerevisiae ATCC 2366, 4 Hansenula anomala R 26,
2 Candida utilis ATCC 9950
All tested yeast strains revealed a limited growth on substrate with pullulan coating.
The inhibitory effect was contained in the range from 14 to 100%. Out of 10 tested
yeast strains, in case of six of them total inhibition of growth on substrate coated with
pullulan coating was observed. This group implied: Endomyces magnusi, Kluyveromy-
ces fragilis R 11, Pichia jadinii, Rhodotorula rubra Rh VIII, Schizosaccharomyces
pombe R 25 and Torulopsis utilis R 10.
In case of the four remaining yeast strains their growth under pullulan coating was
slower in relation to the control culture. The least sensitive to presence of pullulan coat-
ing proved to be the strain Candida utilis ATCC 9950. Its growth was inhibited only
in 14%, which may prove, that this species is little sensitive to unfavourable environ-
mental conditions, into which a limited access of oxygen can be numbered.
Effect of pullulan coating upon speed of growth of chosen bacteria strains
Five strains of Gram (+) bacteria and three strains of Gram(-) bacteria were chosen
for the tests. Part of them reveal oxygen metabolism, the remaining ones are relative
anaerobes. On base of the obtained results for each of the eight tested bacteria strains
inhibition s degree of growth on substrate with pullulan coating in relation to the con-
trolled substrate was calculated. The obtained results were presented on the Figure 3.
Acta Scientiarum Polonorum, Technologia Alimentaria 8(3) 2009
%
44 A. Chlebowska-Åšmigiel, M. Gniewosz
100
90
80
70
60
50
40
30
20
10
0
2 5 7 4 3 8 6 1
Bacteria strains
Fig. 3. The degree of growth s inhibition of chosen bacteria strains through pul-
lulan coating: 2 Citrobacter freundii ATCC 8090, 5 Lactobacillus
plantarum ATCC 4080, 7 Pseudomonas fluorescens, 4 Lactobacillus
brevis, 3 Escherichia coli ATCC 25922, 8 Tetracoccus sp., 6 Micro-
coccus luteus ATCC 9341, 1 Bacillus subtilis ATCC 6650
In case of all tested bacteria strains inhibitory effect of the applied pullulan coating
was observed. The inhibitory effect was diverse for the tested strains and it was from 63
to 100%. The most limited was growth of the strains Citrobacter freundii ATCC 8090,
Lactobacillus plantarum ATCC 4080 and Pseudomonas fluorescens. The least sensitive
to presence of pullulan coating proved to be the strain Bacillus subtilis ATCC 6650.
As it is reported by Burbianka and Pliszka [1983] numerous species of the genus Bacillus
can also reproduce at reduced oxygen pressure, especially in presence of hydrocarbons.
Kandemir et al. [2005] tested film produced on basis of pullulan with supplement of
EDTA·H2O and lysozyme. They observed growth inhibition of the strain Escherichia
coli and lack of inhibitory effect on growth of the strain Lactobacillus plantarum. Still
the results of these tests are not completely comparative with the ones obtained in the
present work, because EDTA and lysozyme included into the film are known antibacte-
rial agents. EDTA has lytic effect upon many G (-) bacteria, on the other hand lysozyme
is an enzyme which decomposes cell wall of Gram (+) bacteria [Mecito lu et al. 2006].
SUMMARY
Pullulan coating applied in these tests revealed big barrier characteristics in relation
to oxygen and carbon dioxide, which had effect upon growth s inhibition of most of the
tested microorganisms, responsible for decay of food. Its additional favourable advan-
tage is that inhibitory effect on growth of microorganisms was obtained without apply-
ing supplementary substances having antimicrobial effect. The results of the carried out
tests suggest, that pullulan coating can be an useful element to ensure microbiological
safety of food, thus to prolong food s durability period.
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Effect of pullulan coating on inhibition of chosen microorganisms growth 45
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WPAYW POWAOKI PULLULANOWEJ NA HAMOWANIE WZROSTU
WYBRANYCH DROBNOUSTROJÓW
Wprowadzenie. Pullulan jest rozpuszczalnym w wodzie polisacharydem wytwarzanym
przez grzyby Aureobasidium pullulans. Glukan ten znalazł zastosowanie do powlekania
produktów żywnościowych. Celem badań było otrzymanie powłoki pullulanowej i spraw-
dzenie jej wpływu na wzrost drobnoustrojów, będących przyczyną psucia się żywności.
Materiały i metody. Zastosowano pullulan wytwarzany przez białego mutanta A. pullu-
lans B-1. Sprawdzano przepuszczalność tlenu i dwutlenku węgla przez film wytworzony
z 10-procentowego wodnego roztworu pullulanu oraz stopień zahamowania wybranych
drobnoustrojów przez powłokę pullulanową utworzoną na powierzchni podłoża wzrosto-
wego.
Wyniki. Stwierdzono małą przepuszczalność gazów przez film pullulanowy oraz znaczne
ograniczenie wzrostu wszystkich badanych drobnoustrojów. Obserwowano całkowite za-
hamowanie wzrostu 21 z 36 badanych szczepów oraz częściowe ograniczenie wzrostu
pozostałych 15 szczepów. Efekt hamujący był zróżnicowany i wynosił od 63 do 100%.
Wnioski. Zastosowana w badaniach powłoka pullulanowa wykazała dużą barierowość
w stosunku do tlenu i dwutlenku węgla, co wpłynęło na hamowanie wzrostu większości
badanych drobnoustrojów, odpowiedzialnych za psucie się żywności.
Słowa kluczowe: powłoka jadalna, pullulan, mikrobiologiczne zanieczyszczenie żywności
Accepted for print Zaakceptowano do druku: 25.06.2009
For citation Do cytowania: Chlebowska-Åšmigiel A., Gniewosz M., 2009. Effect of pullulan
coating on inhibition of chosen microorganisms growth. Acta Sci. Pol., Technol. Aliment. 8(3),
37-46.
www.food.actapol.net
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