Published January 24, 2005
JCB: REPORT
Viral killer toxins induce caspase-mediated
apoptosis in yeast
Jochen Reiter,1 Eva Herker,2 Frank Madeo,2 and Manfred J. Schmitt1
1
Applied Molecular Biology, University of the Saarland, D-66041 Saarbrücken, Germany
2
Institute of Molecular Biosciences, Karl-Franzens University, A-8010 Graz, Austria
n yeast, apoptotic cell death can be triggered by various response and rather cause necrotic, toxin-specific cell
factors such as H2O2, cell aging, or acetic acid. Yeast killing. Studies with yca1 and gsh1 deletion mutants
Icaspase (Yca1p) and cellular reactive oxygen species indicate that ROS accumulation as well as the presence of
(ROS) are key regulators of this process. Here, we show yeast caspase 1 is needed for apoptosis in toxin-treated
that moderate doses of three virally encoded killer toxins yeast cells. We conclude that in the natural environment
(K1, K28, and zygocin) induce an apoptotic yeast cell of toxin-secreting killer yeasts, where toxin concentration
response, although all three toxins differ significantly in is usually low, induction of apoptosis might play an im-
their primary killing mechanisms. In contrast, high toxin portant role in efficient toxin-mediated cell killing.
concentrations prevent the occurrence of an apoptotic cell
Introduction
The production of cytotoxic proteins (killer toxins) is a wide- The finding of cell death with apoptosis-like features in
spread phenomenon among a great variety of yeast genera and is yeast (Madeo et al., 1997) was unexpected, as a unicellular or-
typically associated with the secretion of a protein or glyco- ganism seems to have no advantages in committing suicide.
protein toxin that kills susceptible yeast cells in a two-step Further research in this field demonstrated that in yeast apop-
receptor-mediated manner (Bussey et al., 1990; Magliani et al., totic cell death can be induced by different exogenous and in-
1997; Schmitt and Breinig, 2002). In Saccharomyces cerevisiae, trinsic stresses like H2O2, UV irradiation, acetic acid, cell ag-
three different killer toxins (K1, K2, and K28) have been identi- ing, and high pheromone concentration (Madeo et al., 1999;
fied so far, which are all encoded by cytoplasmic persisting Laun et al., 2001; Ludovico et al., 2001; Severin and Hyman,
double-stranded RNA viruses encoding the unprocessed precursor 2002; Del Carratore et al., 2002; Herker et al., 2004). Similar to
proteins of the secreted / toxins (Tipper and Schmitt, 1991; mammalian apoptosis, reactive oxygen species (ROS) play a
Wickner, 1996). Although most viral killer toxins, like the S. central role in most of these apoptotic scenarios. The similarity
cerevisiae K1 toxin and the Zygosaccharomyces bailii toxin between yeast and mammalian apoptosis was further under-
zygocin, act as ionophores and disrupt cytoplasmic membrane lined by the finding of yeast orthologues of a caspase, a proap-
function by forming cation-specific plasma membrane pores optotic serine protease, AIF, and the transkingdom Bax-inhibi-
(Martinac et al., 1990; Weiler et al., 2002; Breinig et al., 2002; tor BI-1 (Madeo et al., 2002; Chae et al., 2003; Fahrenkrog et
Weiler and Schmitt, 2003), the S. cerevisiae K28 toxin enters al., 2004; Wissing et al., 2004). It was shown that debilitated
susceptible cells by receptor-mediated endocytosis, travels the cells die for the benefit of the whole cell population saving lim-
secretion pathway in reverse, and induces a cell cycle arrest at ited nutrients for healthy cells to enable survival of the whole
the G1/S boundary (Schmitt et al., 1996; Eisfeld et al., 2000). population (Fabrizio et al., 2004; Herker et al., 2004). Another
In higher multicellular organisms, it is well known that natural cell death situation for yeast is the exposure to killer
pore-forming toxins like Staphylococcus aureus toxin and/or toxins produced and secreted by concurring killer strains.
inhibitors of protein synthesis like diphtheria toxin produced Therefore, we investigated if killer toxins are able to induce the
and secreted by Corynebacterium diphtheriae are able to induce apoptotic process and if apoptosis is responsible for cell death
apoptosis (Weinrauch and Zychlinsky, 1999). under natural environmental conditions in the presence of mod-
erate or low toxin concentrations closely reflecting the situa-
tion in the natural yeast habitat.
Correspondence to Manfred J. Schmitt: mjs@microbiol.uni-sb.de
Using three viral killer toxins that either disrupt cytoplas-
Abbreviations used in this paper: DHR, dihydrorhodamine; MBA, methylene
blue agar plates; PS, phosphatidylserine; ROS, reactive oxygen species. mic membrane function or arrest cells at the G1/S boundary of
© The Rockefeller University Press $8.00
The Journal of Cell Biology, Vol. 168, No. 3, January 31, 2005 353 358
http://www.jcb.org/cgi/doi/10.1083/jcb.200408071
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THE
JOURNAL
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Figure 1. Yeast virus toxins kill susceptible cells in a dose-dependent manner by inducing either apoptotic or necrotic pathways. (A) TUNEL reaction,
fluorescence and the corresponding phase-contrast display, and DAPI staining after 10-h treatment of S. cerevisiae 192.2d at 20 C at moderate concen-
trations in the range of 6 pmol each of K1, K28, zygocin, heat-inactivated K1 toxin (negative control), and heat-inactivated K1 toxin plus 10-min DNase I
treatment (positive control). (B) Detection of PS exposure via Annexin V staining in S. cerevisiae wild-type cells (strain 192.2d) treated for 10 h at 20 C
with or without killer toxin K28 (6 pmol) and propidium-iodide staining. Bar, 5 m. (C) Kinetics of cell survival of S. cerevisiae 192.2d (wild type) in the
presence of moderate K1 toxin concentrations (6 pmol). (D) Kinetics of cell survival of S. cerevisiae 192.2d (wild type) in the presence of high K1 toxin con-
centrations (12 pmol). (E) TUNEL staining of wild-type cells of S. cerevisiae 192.2d after treatment with high concentrations of K1 toxin (12 pmol). Bar, 5 m.
(F) Dose response relationship between yeast cell survival and apoptotic cell response (determined by TUNEL staining) of S. cerevisiae 192.2d in depen-
dence of increasing K1 toxin concentrations (each experiment was initiated with 1.1 106 colony forming units/ml). At the indicated K1 toxin concentration,
the frequency of TUNEL-positive cells was determined for at least 400 cells in three independent experiments.
the eukaryotic cell cycle, we found that all toxins induce cell In contrast, high concentrations of all three toxins led to nonap-
death in S. cerevisiae when added in moderate or low concen- optotic cell death independent of yeast caspase 1 and ROS.
trations, always accompanied by the production of ROS, DNA
fragmentation, typical phenotypic changes, and translocation
Results and discussion
of phosphatidylserine (PS) from the inner to the outer leaflet of
the cytoplasmic membrane the defining phenotype of apop- Killer toxins can induce both apoptotic
and necrotic cell death in yeast
totic cell death. A yeast yca1 disruptant showed significantly
decreased toxin sensitivity, whereas yeast mutants blocked Treatment of yeast cells with low concentrations of three dif-
in endogenous glutathione biosynthesis were hypersensitive. ferent viral killer toxins resulted in a moderate rate of cell death
354 JCB " VOLUME 168 " NUMBER 3 " 2005
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Table I. Major properties of the viral killer toxins K1, K28, and zygocin
Toxin Size Structure Receptor on target cell Lethal effect
D
K1 19,088 / heterodimer -1,6-D-glucan ionophore
K28 21,496 / heterodimer -1,6-mannoprotein G1/S cell cycle arrest
Zygocin 10,421 monodimer -1,6-mannoprotein ionophore
Data for K1, K28, and zygocin were taken from Bostian et al. (1984), Schmitt and Tipper (1995), and Weiler et al. (2002), respectively (adapted from Breinig et al., 2004).
ROS mediate cell death in killer toxin
(Fig. 1 C). We tested wild-type yeast cells (strain 192.2d)
treated cells
treated under these conditions for typical apoptotic markers by
TUNEL assays and DAPI staining. After 10 h of treatment with ROS play a central role in inducing apoptotic markers and me-
either K1, K28, or zygocin, a strong green fluorescence indicat- diating cell death in yeast (Madeo et al., 1999; Laun et al.,
ing DNA fragmentation could be detected, whereas negative 2001; Ludovico et al., 2001; Mazzoni et al., 2003; Weinberger
control cells treated with heat-inactivated killer toxin did not et al., 2003). We incubated cells with dihydrorhodamine
show any DNA fragmentation (Fig. 1 A). The fluorescence was (DHR) 123, which is a cell permeable leukodye that converts to
located in the nucleus, as could be proven by DAPI counter a red light emitting fluorochrome in the presence of ROS. After
staining (Fig. 1 A). Furthermore, DAPI staining revealed an 10 h of killer toxin treatment, an intensive red fluorescence
atypical nuclear phenotype with condensed chromatin in the could be detected for all three killer toxins (Fig. 2 A), whereas
toxin-treated samples (Fig. 1 A). Thus, all three killer toxins no ROS were produced in negative control cells that had been
tested likewise induce genomic DNA fragmentation and chro- treated with heat-inactivated toxin (Fig. 2 A). To determine the
matin condensation, although their primary mode of action and initial time point when ROS first appeared, samples of K1-
mechanism of cell killing differs significantly (Table I). treated cells were taken at 1-h intervals. A weak signal ap-
In contrast, treatment with high concentrations of killer peared after 2 h of toxin treatment, which became significantly
toxins resulted in fast cell killing (Fig. 1 D) resembling necrosis more intense thereafter. The majority of the cells (99%) were
as DNA fragmentation could not be detected (Fig. 1 E). To de- stained within two cell generations ( 8 h), whereas in the con-
termine if an in vivo breakpoint exists at which efficient cell trol (heat-inactivated toxin) only 1% of the cells were fluorescent
killing cuts in while apoptotic cell responses simultaneously dis- (unpublished data).
appear, a dose response curve was created that correlates yeast Interestingly, in phase contrast of K28-treated cells we
cell survival with the appearance of TUNEL-positive apoptotic could observe an apoptosis-typical shrinking and condensation
cells in dependence of increasing K1 toxin concentrations. As il- of only those cells that showed a positive staining for ROS
lustrated in Fig. 1 F, the K1 virus toxin caused a rapid cell kill- (Fig. 2 B). After incubation with higher doses of the toxins,
ing as well as a TUNEL-positive yeast cell response, both con- which resulted in faster killing kinetics (0.5 1 h), cells died
tinuously increasing until K1 reached molar concentrations of without accumulation of ROS (Fig. 2 C).
up to 6 pmol. At higher concentrations (8 and 12 pmol K1), ne- To further analyze the role of intracellular ROS in toxin-
crotic cell killing dramatically increased, whereas the percent- induced cell death, we tested yeast gsh1 mutant cells that are
age of TUNEL-positive, apoptotic cells steadily declined down genetically blocked in glutathione biosynthesis. Glutathione
to 12% (Fig. 1 F). Thus, in the case of the K1 virus toxin, a con- acts as a redox buffer and protects cells from damage by reduc-
centration close to 6 pmol resembles the breakpoint at which ing the amount of ROS (Carmody and Cotter, 2001). Toxin
efficient necrotic cell killing occurs, whereas apoptotic cell sensitivity assays determined in the well-test on methylene
responses are of minor importance for in vivo toxicity. blue agar plates (MBA assay) indicated that gsh1 mutant cells
Translocation of PS from the inner leaflet to the extracel- were significantly more sensitive against killer toxin treatment
lular side of the plasma membrane is an early event in apopto- than the isogenic wild type and displayed a considerably larger
sis and can be detected by Annexin V, a protein with strong af- zone of growth inhibition (Fig. 2 D). Considering the linear re-
finity to PS (Martin et al., 1995). To demonstrate that yeast cell lationship between the diameter of the inhibition zone and the
spheroplasts are still intact, a propidium iodide staining was logarithm of the killer toxin concentration, the hypersensitive
performed. Because both zygocin and K1 kill their correspond- phenotype of gsh1 mutant cells portrays the evident involve-
ing target cell by disruption of plasma membrane integrity, al- ment of ROS in toxin-induced cell death.
most all cells were stained with propidium iodide (unpublished In addition, experiments in liquid medium confirmed the
data), and, therefore, Annexin V staining was not performed on central role of ROS in toxin-mediated cell killing as toxin-
cells treated with these toxins. In contrast, K28 has no effect on treated gsh1 cells showed a more intense DHR 123 staining
membrane integrity as it irreversibly inhibits nuclear DNA syn- (not depicted) and were killed more effectively than the
thesis and arrests cells at the G1/S boundary of the cell cycle isogenic wild type (Fig. 2 E). The time course of cell survival
(Schmitt et al., 1996). Indeed, only 10% of K28-treated cells was reproduced in three independent experiments. Supple-
had taken up propidium iodide and 20% of the cells were PS mentation of the growth medium with 20 l/ml of reduced
positive, showing PS translocation to the outer leaflet of the glutathione (50 mM) protected the cells to some extent, and
plasma membrane (Fig. 1 B). after 24 h killer toxin treatment cell survival rate in glutathione-
KILLER TOXINS INDUCE APOPTOSIS IN YEAST " REITER ET AL. 355
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Figure 2. ROS mediate apoptotic cell death induced by killer toxins. (A) DHR staining and corresponding phase contrast of S. cerevisiae 192.2d (wild type)
treated for 10 h at 20 C with moderate concentrations of either K1, K28, or zygocin (6 pmol each). Samples of heat-inactivated toxin were used as
negative control. Bar, 5 m. (B) Phase contrast and DHR staining of K28-treated cells and phase contrast of negative control cells treated with heat-inactivated
K28 toxin. Bar, 5 m. (C) DHR staining of S. cerevisiae 192.2d (wild type) after treatment with high concentrations of K1 toxin (12 pmol). Bar, 5 m.
(D) Toxin sensitivity assay on MBA illustrating K1 hypersensitivity of yeast gsh1 mutant cells compared with its isogenic Gsh1 wild type. (E) Kinetics of
cell survival of a yeast gsh1 mutant and its isogenic Gsh1 wild type under moderate K1 toxin concentrations (6 pmol).
supplemented yeast cultures was increased by a factor of cell survival was reproduced in three independent experiments.
two (unpublished data). These results suggest that ROS act as Furthermore, the occurrence of apoptotic markers was strongly
effectors of apoptosis in toxin-treated cells and trigger the reduced compared with wild type (Fig. 3 B, exemplarily shown
subsequent mechanisms. for K1), indicating that Yca1p is required for the efficient oc-
currence of apoptotic markers. Residual cell killing seen in the
Toxin-mediated apoptotic cell killing is
yca1 mutant after K1 toxin treatment at the 6-pmol level is
dependent on YCA1
caused by the toxin s primary lethal effect, which can be par-
Recently, a caspase homologue was identified in yeast that was tially separated from the apoptosis effects in a dose-dependent
shown to mediate apoptosis in this unicellular microorganism manner (as shown in section Killer toxins can induce both apop-
(Uren et al., 2000; Madeo et al., 2002). We analyzed the in- totic and necrotic cell death in yeast; Fig. 1 F). Next, we simu-
volvement of Yca1p in killer toxin action and cell death. Dele- lated the natural environment of yeast, where toxin concentra-
tion of YCA1 had only little effect on toxin sensitivity. In MBA tion is usually low, by application of K28 in a significantly
sensitivity assays under high toxin concentration, the resulting lower concentration of 1 pmol, corresponding to only 32 ng of
growth inhibition zones induced by either K1 or K28 did not the purified protein toxin. Under these conditions, deletion of
differ significantly from the basal sensitivity of the isogenic YCA1 strongly reduced toxin sensitivity; although the deletion
Yca1 wild type (unpublished data). However, in liquid me- mutant continued to proliferate even during toxin treatment for
dium, the yca1 deletion mutant displayed a slightly better sur- 20 h, cell growth in the Yca1 wild type ceased and viable cell
vival compared with wild type (Fig. 3 A). The time course of numbers remained constant (Fig. 3 C).
Figure 3. Yeast caspase 1 is required for an efficient apoptotic cell response against the K28 virus toxin. (A) Kinetics of cell survival of a yeast yca1 null
mutant and its isogenic Yca1 wild type (strain 192.2d) in the presence of moderate K28 toxin concentrations (6 pmol). (B) DHR and TUNEL staining of
yca1 mutant cells and its isogenic wild type (strain 192.2d) after treatment with moderate concentrations of K1 toxin (6 pmol). Bars, 5 m. (C) Kinetics
of cell survival of a yeast yca1 mutant and its isogenic wild type (strain 192.2d) under low K28 toxin concentrations (1 pmol).
356 JCB " VOLUME 168 " NUMBER 3 " 2005
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Table II. S. cerevisiae strains used in this study
Strain Genotype Reference
192.2d MAT ura3 leu2 Schmitt et al., 1996
192.2d yca1 MAT ura3 leu2 YOR197w::kanMX4 This study
YPH98 MATa ura3-52 lys2-801 ade2-101 leu2-3, 112 trp1- 1 Sikorski and Hieter, 1989
YPH98 gsh1 MATa gsh1::URA3 ura3-52 lys2-801 ade2-101 leu2-3, 112 trp1- 1 Brendel et al., 1998
a final concentration of 1 12 pmol, and cell survival was determined by
Based on the data presented here, we could show that in
plating aliquots of appropriately diluted cell suspensions onto YEPD agar
low concentrations all three virally encoded yeast toxins induce
plates containing 0.6 M KCl. After incubating the plates for 3 d at 30 C,
apoptotic cell death that is accompanied by DNA fragmenta-
colony-forming units were determined on plates with at least 400 cells.
tion, chromatin condensation, and (as shown for K28) PS ex-
Detection of apoptotic phenotypes
ternalization. This process is mediated through yeast caspase
To detect an apoptotic phenotype, cells were analyzed by TUNEL test (In
Yca1p and the generation of ROS. In contrast, high concentra-
Situ Cell Death Detection Kit, POD; Roche), Annexin V staining (ApoAlert
AnnexinV Apotosis kit; CLONTECH Laboratories, Inc.), DAPI staining (in-
tions of killer toxins induce nonapoptotic necrotic cell death,
cubation with 1 mg/ml DAPI), and DHR staining as described previously
which is independent of Yca1p and ROS. Therefore, killer
(Madeo et al., 1997, 1999). Fluorescent light microscopy involved a mi-
toxin action can trigger two modes of cell death. Under high
croscope (model BX5; Olympus) with GFP, DAPI, and Rhodamine filters
under standard settings.
toxin concentrations induction of apoptosis plays a minor role,
whereas under moderate or low toxin doses, resembling the in
Disruption of YCA1
vivo situation in the natural habitat of killer yeasts (Starmer et
A disruption cassette consisting of a geneticin resistance gene (KanR) with
flanking 45 bases of 5 and 3 sequences of the YCA1 ORF was PCR
al., 1987), it might be of general importance for a toxin-secret-
generated with primer YcaDelUp (5 -CGGGTAATAACAACTATTGAA-
ing yeast to induce apoptosis in competing yeast cells, in par-
AAAGCATGGCTTCGCATTAATAGGTTCGTACGCTGCAGGTCGAC-3 )
ticular at toxin concentrations that are per se too low to kill via
and YCADelDown (5 -CGTTAAAAAAACACATGGTCTTATTTTCCAAAAT-
GCCTATTCCCCCACTAGTGGATCTGATATC-3 ) and transformed into the
the toxin s primary mode of action.
toxin-sensitive S. cerevisiae strain 192.2d. Geneticin-resistant colonies
were confirmed for correct disruption by PCR with three primers
(YCA1DelTestf, 5 -CAGTTCTCCTTAAAATCCACATAA-3 ; YCADelTestr,
Materials and methods
5 -GTCGAAACAAGAAGAGCAAAC-3 ; KanRTestr, 5 -AAACAGGAATC-
GAATGCAACC-3 ).
Strains
S. cerevisiae strains used throughout this work are listed in Table II. Exper-
Reproducibility of the results
iments with yca1 mutant cells and their isogenic wild-type strains were
All experiments were repeated at least three times. Quantitative data from
performed in two different strain backgrounds with similar results. Data
TUNEL tests, ROS staining, and Annexin V staining are from one represen-
shown in this paper were performed with the toxin-sensitive tester strain S.
tative experiment, whereby at least 400 cells were counted.
cerevisiae 192.2d (Schmitt et al., 1996) and its isogenic knockout mu-
tants. Yeast cultures were grown at 20 C in complex YPC medium, which
We are grateful to Beate Schmitt for technical assistance and to Frank Breinig
corresponds to YEPD medium supplemented with 1.92% citric acid; pH
for helpful discussion and critical reading of the manuscript.
was adjusted to 4.7 by the addition of K2HPO4 as previously described
This work was kindly supported by grants from the Deutsche For-
(Riffer et al., 2002).
schungsgemeinschaft to M.J. Schmitt (Schm 541/11-1 and Schm 541/10-1)
and to E. Herker and F. Madeo.
Toxin production and killer assay
Killer toxins K1, K28, and zygocin were isolated and partially purified
Submitted: 11 August 2004
from cell-free culture supernatants of the killer yeasts S. cerevisiae strain
Accepted: 13 December 2004
K7 (K1 toxin), strain MS300c (K28 toxin), and Zygosaccharomyces bailii
strain 412 (zygocin toxin) as previously described (Schmitt and Tipper,
1990; Schmitt and Neuhausen, 1994; Weiler and Schmitt, 2003). To de-
termine toxin-specific cell killing, agar diffusion assays on MBA, pH 4.7,
References
were performed against a lawn of 105 cells of the toxin-sensitive yeast
strains 192.2d, YPH98, or YPH98 gsh1 (Schmitt and Tipper, 1995). To
Bostian, K.A., H. Bussey, Q. Elliot, V. Burn, A. Smith, and D.J. Tipper. 1984.
quantify activity and concentration in each of the three virus toxin prepara-
Sequence of the preprotoxin dsRNA gene of type I killer yeast: multiple
tions, 0.1-ml toxin samples were pipetted into wells (10 mm in diameter) processing events produce a two-component toxin. Cell. 36:741 751.
cut into the agar, and the plates were incubated for 4 d at 20 C as previ-
Breinig, F., D.J. Tipper, and M.J. Schmitt. 2002. Kre1p, the plasma membrane
ously described (Schmitt and Tipper, 1990). The diameter of the growth-
receptor for the yeast K1 viral toxin. Cell. 108:395 405.
free zone around the wells is proportional to the logarithm of the killer
Breinig, F., K. Schleinkofer, and M.J. Schmitt. 2004. Yeast Kre1p is GPI-
toxin activity, which is expressed in arbitrary units; 1,000 arbitrary units
anchored and involved in both cell wall assembly and architecture.
each of K1, K28, and zygocin correspond to 0.3, 1.0, and 0.4 g of the
Microbiology. 150:3209 3218.
purified killer toxin, respectively. In the present study, low, moderate, and
Brendel, M., M. Grey, A.F. Maris, J. Hietkamp, Z. Fesus, C.T. Pich, L. Dafre,
high toxin concentrations are defined as 1, 6, and 12 pmol each of the
M. Schmidt, F. Eckardt-Schupp, and J.A.P. Henriques. 1998. Low glu-
three virus toxins, corresponding to protein concentrations of 30 ng (K1),
tathione pools in the original pso3 mutant of Saccharomyces cerevisiae
32 ng (K28), and 16 ng (zygocin) that were used in the experiments in are responsible for its pleiotropic sensitivity phenotype. Curr. Genet.
33:4 9.
which the in vivo killing kinetics of each toxin had been determined.
Bussey, H., C. Boone, H. Zhu, T. Vernet, M. Whiteway, and D.Y. Thomas.
Killing kinetics 1990. Genetic and molecular approaches to synthesis and action of the
yeast killer toxin. Experientia. 46:193 200.
To examine killer toxin activity in liquid media, yeast strains were incu-
bated under shaking at 30 C in YPC medium, pH 4.7, until cells had
Carmody, R.J., and T.G. Cotter. 2001. Signalling apoptosis: a radical approach.
reached early exponential growth phase (2 107 cells/ml). Thereafter, Redox Rep. 6:77 90.
cells were spheroplasted by treatment with Zymolyase 20T as described
Chae, H.J., N. Ke, H.R. Kim, S. Chen, A. Godzik, M. Dickman, and J.C. Reed.
previously (Eisfeld et al., 2000) and incubated in osmotically stabilized
2003. Evolutionarily conserved cytoprotection provided by Bax Inhibitor-1
medium containing 0.6 M KCl. The appropriate killer toxin was added to homologs from animals, plants, and yeast. Gene. 323:101 113.
KILLER TOXINS INDUCE APOPTOSIS IN YEAST " REITER ET AL. 357
Downloaded from jcb.rupress.org on June 15, 2011
Published January 24, 2005
Del Carratore, R., C.C. Della, M. Simili, E. Taccini, M. Scavuzzo, and S. Weiler, F., and M.J. Schmitt. 2003. Zygocin, a secreted antifungal toxin of the
Sbrana. 2002. Cell cycle and morphological alterations as indicative of yeast Zygosaccharomyces bailii, and its effect on sensitive fungal cells.
apoptosis promoted by UV irradiation in S. cerevisiae. Mutat. Res. FEMS Yeast Res. 3:69 76.
513:183 191.
Weiler, F., K. Rehfeldt, F. Bautz, and M.J. Schmitt. 2002. The Zygosaccharo-
Eisfeld, K., F. Riffer, J. Mentges, and M.J. Schmitt. 2000. Endocytotic uptake myces bailii antifungal virus toxin zygocin: cloning and expression in a
and retrograde transport of a virally encoded killer toxin in yeast. Mol. heterologous fungal host. Mol. Microbiol. 46:1095 1105.
Microbiol. 37:926 940.
Weinberger, M., L. Ramachandran, and W.C. Burhans. 2003. Apoptosis in
Fabrizio, P., L. Battistella, R. Vardavas, C. Gattazzo, L.L. Liou, A. Diaspro, yeasts. IUBMB Life. 55:467 472.
J.W. Dossen, E.B. Gralla, and V.D. Longo. 2004. Superoxide is a mediator
Weinrauch, Y., and A. Zychlinsky. 1999. The induction of apoptosis by bacte-
of an altruistic aging program in Saccharomyces cerevisiae. J. Cell Biol.
rial pathogens. Annu. Rev. Microbiol. 53:155 187.
166:1055 1067.
Wickner, R.B. 1996. Double-stranded RNA viruses of yeast. Microbiol. Rev.
Fahrenkrog, B., U. Sauder, and U. Aebi. 2004. The S. cerevisiae HtrA-like pro-
60:250 265.
tein Nma111p is a nuclear serine protease that mediates yeast apoptosis.
Wissing, S., P. Ludovico, E. Herker, S. Buttner, S.M. Engelhardt, T. Decker, A.
J. Cell Sci. 117:115 126.
Link, A. Proksch, F. Rodrigues, M. Corte-Real, et al. 2004. An AIF
Herker, E., H. Jungwirth, K.A. Lehmann, C. Maldener, K.U. Frohlich, S. Wis-
orthologue regulates apoptosis in yeast. J. Cell Biol. 166:969 974.
sing, S. Buttner, M. Fehr, S. Sigrist, and F. Madeo. 2004. Chronological
aging leads to apoptosis in yeast. J. Cell Biol. 164:501 507.
Laun, P., A. Pichova, F. Madeo, J. Fuchs, A. Ellinger, S. Kohlwein, I. Dawes,
K.U. Frohlich, and M. Breitenbach. 2001. Aged mother cells of Saccha-
romyces cerevisiae show markers of oxidative stress and apoptosis.
Mol. Microbiol. 39:1166 1173.
Ludovico, P., M.J. Sousa, M.T. Silva, C. Leao, and M. Corte-Real. 2001. Sac-
charomyces cerevisiae commits to a programmed cell death process in
response to acetic acid. Microbiology. 147:2409 2415.
Madeo, F., E. Frohlich, and K.U. Frohlich. 1997. A yeast mutant showing diag-
nostic markers of early and late apoptosis. J. Cell Biol. 139:729 734.
Madeo, F., E. Frohlich, M. Ligr, M. Grey, S.J. Sigrist, D.H. Wolf, and K.U.
Frohlich. 1999. Oxygen stress: a regulator of apoptosis in yeast. J. Cell Biol.
145:757 767.
Madeo, F., E. Herker, C. Maldener, S. Wissing, S. Lachelt, M. Herlan, M. Fehr, K.
Lauber, S.J. Sigrist, S. Wesselborg, and K.U. Frohlich. 2002. A caspase-
related protease regulates apoptosis in yeast. Mol. Cell. 9:911 917.
Magliani, W., S. Conti, M. Gerloni, D. Bertolotti, and L. Polonelli. 1997. Yeast
killer systems. Clin. Microbiol. Rev. 10:369 400.
Martin, S.J., C.P. Reutelingsperger, A.J. McGahon, J.A. Rader, R.C. van Schie,
D.M. LaFace, and D.R. Green. 1995. Early redistribution of plasma
membrane phosphatidylserine is a general feature of apoptosis regardless
of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl.
J. Exp. Med. 182:1545 1556.
Martinac, B., H. Zhu, A. Kubalski, X.L. Zhou, M. Culbertson, H. Bussey, and
C. Kung. 1990. Yeast K1 killer toxin forms ion channels in sensitive
yeast spheroplasts and in artificial liposomes. Proc. Natl. Acad. Sci. USA.
87:6228 6232.
Mazzoni, C., P. Mancini, L. Verdone, F. Madeo, A. Serafini, E. Herker, and C.
Falcone. 2003. A truncated form of KlLsm4p and the absence of factors
involved in mRNA decapping trigger apoptosis in yeast. Mol. Biol. Cell.
14:721 729.
Riffer, F., K. Eisfeld, F. Breinig, and M.J. Schmitt. 2002. Mutational analysis of
K28 preprotoxin processing in the yeast Saccharomyces cerevisiae. Mi-
crobiology. 148:1317 1328.
Schmitt, M.J., and D.J. Tipper. 1990. K28, a unique double-stranded RNA killer
virus of Saccharomyces cerevisiae. Mol. Cell. Biol. 10:4807 4815.
Schmitt, M.J., and F. Neuhausen. 1994. Killer toxin-secreting double-stranded
RNA mycoviruses in the yeasts Hanseniaspora uvarum and Zygosac-
charomyces bailii. J. Virol. 68:1765 1772.
Schmitt, M.J., and D.J. Tipper. 1995. Sequence of the M28 dsRNA: preprotoxin is
processed to an / heterodimeric protein toxin. Virology. 213:341 351.
Schmitt, M.J., and F. Breinig. 2002. The viral killer system in yeast: from mo-
lecular biology to application. FEMS Microbiol. Rev. 26:257 276.
Schmitt, M.J., P. Klavehn, J. Wang, I. Schonig, and D.J. Tipper. 1996. Cell cy-
cle studies on the mode of action of yeast K28 killer toxin. Microbiology.
142:2655 2662.
Severin, F.F., and A.A. Hyman. 2002. Pheromone induces programmed cell
death in S. cerevisiae. Curr. Biol. 12:R233 R235.
Sikorski, R.S., and P. Hieter. 1989. A system of shuttle vectors and yeast host
strains designed for efficient manipulation of DNA in Saccharomyces
cerevisiae. Genetics. 122:19 27.
Starmer, W.T., P.F. Ganter, V. Aberdeen, M. Lachance, and H.J. Phaff. 1987.
The ecological role of killer yeasts in natural communities of yeasts.
Can. J. Microbiol. 33:783 796.
Tipper, D.J., and M.J. Schmitt. 1991. Yeast dsRNA viruses: replication and
killer phenotypes. Mol. Microbiol. 5:2331 2338.
Uren, A.G., K. O Rourke, L.A. Aravind, M.T. Pisabarro, S. Seshagiri, E.V.
Koonin, and V.M. Dixit. 2000. Identification of paracaspases and meta-
caspases: two ancient families of caspase-like proteins, one of which
plays a key role in MALT lymphoma. Mol. Cell. 6:961 967.
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