117828259

TLC-MS VERSUS TLC-LC-MS FINGERPRINTS OF HERBAL EXTRACTS. PART I. ESSENTIAL OILS

Mieczysław Sajewicz¹, Łukasz Wojtal¹, Maja Natić¹’², Dorota Staszek¹, Monika Waksmundzka-Hajnos³, and Teresa Kowalska¹*

¹ Institute of Chemistry, University of Silesia, 9 Szkolna Street, 40-006 Katowice, Poland ²Faculty of Chemistry, University of Belgrade, Studentski Trg 12-16, 11000 Belgrade, Serbia ³Deaprtment of Inorganic Chemistry, Medical University of Lublin, 6 Staszica Street, 20-081 Lublin, Poland

Abstract: In our earlier studies, we proposed the low-temperature thin-layer chromatography with densitometric and mass spectrometric detection (LT TLC-densitometry and LT TLC-MS, respectively) for fingerprinting of essential oils derived from several different species from the sagę (Salvia) genus (and morę generally, for fingerprinting of the complex mixtures of organie compounds of the natural, or synthetic origin). Development of the proposed LT-TLC-MS approach was only possible with use of the TLC-MS interface able to couple the developed chromatographic piąte with mass spectrometer. The goal of this study was to develop for the same purpose the two-dimensional liquid chromatographic system and to examine its performance with the sagę sample. Development of such a system was only possible with use of the TLC-MS interface and it included the low-temperature thin-layer chromatography coupled off-line with high-performance liquid chromatography, to build an overall LT TLC-LC-MS system. We compared the fingerprinting results for the essential oil of the test plant (Sahia lavandulifolia) obtained with use of the earlier proposed one-dimensional system (ID LT TLC-MS) and the results obtained with use of the two-dimensional system (2D LT TLC-LC-MS), newly proposed in this study. It was shown that the 2D approach to the separation of a complex mixture of botanical origin provides morę fingerprints for further investigations than the ID approach. Moreover, it is also elear that the 2D approach is better suited for identification of individual Chemical species contained in a given mixture, due to the simpler pattems of mass spectra obtained from the 2D analytical modę than from the ID one. However, with the constituents of the essential oil derived from Sahia lavandulifolia, a straightforward identification was not possible, basically due to

Wyszukiwarka

Podobne podstrony:
Low-Temperature TLC-MS of Essential Oils from Five Different Sagę (Sa/wa) Species Mieczysław Sajewlc
Low-Temperature TLC-MS of Essential Oils(1) 275 20)
Low Temperalure TLC-MS of Essentiai Oils 200
Essential Oils of Sagę Species 187 Fig. 1. Fingerprint gas chromalograms obtained from HS-GC-MS anal
—SENTE MAKlNA lN§AAT SANAYl ve TlC.LTD.ęTf.CERTIFICATE OF THE EXC LUSIYE DEALER We declare and confi
660 examples of?ms examples of damsejemplosM de presasF
661 examples of?ms examples of dams examples of dams cross section of an arch dam secciónFtransver
MS W. Number of European bison in Roland and worldwideLABOKLIN poun m < tto unmui uaancM ^€MVTCX.
Ada Chromatographica 21(2009)3, 1-18 DOI: 10.1556/AChrom.21.2009.3.9 GC-MS Study of the Performance
9 preliminarily separated by means of LT TLC (see Fig. l(o)). Each of these three figures shows the
f Rubberpad B C IfnlrrW] [ms] Pług of axoplasm I Perfusion fluid D / Cut end / Pług of
—SENTE MAKlNA lN§AAT SANAYl ve TlC.LTD.ęTf.CERTIFICATE OF THE EXC LUSIYE DEALER We declare and confi
Shuttle Tatting Without A Teacher (6) the loop which was formed over the fingers of your right hand.
/jUźżł^CU^ jyU Ij i, jy : COPY    i .li] , , 4Aj - v »v lc 1r Ukaz of June 22.’.id,
fickle finger of?te Fickie Finger of Fate łrrdćOtkv> » the key to ti&ńbitity The targel Mum
s&h 109 100 STRKNGTH AND HEALTH THE AH.MS—Nd. 3 Take a firm grip willi oue band of tbe fingers of ll

więcej podobnych podstron